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  • BSI
    BS EN ISO 6579-4:2025 Microbiology of the food chain. Horizontal method for the detection, enumeration and serotyping of Salmonella - Identification of monophasic Salmonella Typhimurium (1,4,[5],12:i:-) by polymerase chain reaction (PCR)
    Edition: 2025
    $455.10
    / user per year

Description of BS EN ISO 6579-4:2025 2025

This document specifies a horizontal in vitro method for the molecular identification and differentiation of the monophasic variant of Salmonella enterica subsp. enterica serovar Typhimurium (1,4,[5],12:i:-) lacking the second H phase H:1,2, starting from isolates. The method detects specific DNA sequences of an intergenic region of the first H phase flagellin cluster for identification of Salmonella enterica subsp. enterica serovar Typhimurium (further called Salmonella Typhimurium) and specific DNA sequences of genes associated with second H phase flagellar antigen expression. The method is applicable for: —     differentiation of the isolate under analysis between monophasic Salmonella Typhimurium and the monophasic variant of another Salmonella non-Typhimurium serovar that has the same antigenic formula; —     identification of the isolate under analysis being either monophasic Salmonella Typhimurium or (biphasic) Salmonella Typhimurium. This document is applicable for the analysis of a pure culture belonging to the genus Salmonella, isolated from: —     products intended for human consumption; —     products intended for animal feeding; —     environmental samples in the area of food and feed production and handling; —     samples from the primary production stage. This document can also be applied in other domains for identification of monophasic Salmonella Typhimurium (e.g. environmental, human health, animal health). NOTE            This method has been validated in a method evaluation study and in an interlaboratory study with a large set of different strains (target and non-target strains), isolated from different sources (food products, animals, animal feed, primary production samples and humans). For detailed information on the validation, see Annex E.

Standard NumberBS EN ISO 6579-4:2025
TitleMicrobiology of the food chain. Horizontal method for the detection, enumeration and serotyping of Salmonella - Identification of monophasic Salmonella Typhimurium (1,4,[5],12:i:-) by polymerase chain reaction (PCR)
StatusDefinitive
Publication Date2025-02-07
Identical National Standard OfEN ISO 6579-4, ISO 6579-4
DescriptorsMicrobiology, Food, Food control, Food manufacturing processes, Food technology, Food testing, Contaminant determination (food), Enumeration, Salmonella
ICS07.100.30 Food microbiology
CommitteeAW/9
ISBN978 0 539 29261 9
PublisherBSI
Pages42



About BSI

BSI Group, also known as the British Standards Institution is the national standards body of the United Kingdom. BSI produces technical standards on a wide range of products and services and also supplies certification and standards-related services to businesses.
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