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  • BSI
    BS EN 18033:2024 Food authenticity. Quantitation of equine DNA relative to mammalian DNA in raw beef (meat)
    Edition: 2025
    $341.37
    / user per year

Description of BS EN 18033:2024 2025

This document specifies a real-time PCR procedure for the quantitation of the amount of equine DNA relative to total mammalian DNA in a raw meat sample. Results of this equine assay are expressed in terms of equine (Equus genus) haploid genome copy numbers relative to total mammalian haploid genome copy numbers. This assay is specific for representatives of the genus Equus and therefore detects horse, mule, donkey and zebra DNA. The method has been previously validated in a collaborative study and applied to DNA extracted from samples that consist of raw horse meat in a raw beef (meat) background. The limit of detection has been determined experimentally to be at least 17 horse haploid genome equivalents (HGE) for both the equine PCR and the mammalian PCR based on the lowest dilution on the respective calibration curves through single laboratory validation. The lowest relative horse content of the target sequence included in the collaborative study was a mass fraction of 0,1 % based on gravimetrically prepared raw horse muscle tissue in a raw beef muscle tissue background. The compliance assessment process is not part of this document.

Standard NumberBS EN 18033:2024
TitleFood authenticity. Quantitation of equine DNA relative to mammalian DNA in raw beef (meat)
StatusDefinitive
Publication Date2025-01-13
Identical National Standard OfEN 18033
DescriptorsFood, Food technology, Food testing, DNA, Meat, Beef
ICS07.100.30 Food microbiology, 67.120.10 Meat and meat products
CommitteeAW/34/46
ISBN978 0 539 28583 3
PublisherBSI
Pages16



About BSI

BSI Group, also known as the British Standards Institution is the national standards body of the United Kingdom. BSI produces technical standards on a wide range of products and services and also supplies certification and standards-related services to businesses.
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