1. Scope

1.1 This guide covers procedures for obtaining laboratory data concerning the adverse effects of a test material added to growth medium on growth of certain species of freshwater and saltwater microalgae during a static exposure. These procedures will probably be useful for conducting short-term toxicity tests with other species of algae, although modifications might be necessary. Although the test duration is comparable to an acute toxicity test with aquatic animals, an algal toxicity test of short duration (72, 96 or 120 h) allows for examination of effects upon multiple generations of an algal population and thus should not be viewed as an acute toxicity test.

1.2 Other modifications of these procedures might be justified by special needs or circumstances. Although using appropriate procedures is more important than following prescribed procedures, results of tests conducted using unusual procedures are not likely to be comparable to results of many other tests. Comparison of results obtained using modified and unmodified versions of these procedures might provide useful information concerning new concepts and procedures for conducting toxicity tests with microalgae.

1.3 These procedures are applicable to many chemicals, either individually or in formulations, commercial products, or known mixtures. With appropriate modifications, these procedures can be used to conduct tests on temperature, and pH and on such materials as aqueous effluents (see also Guide E 1192), leachates, oils, particulate matter, sediments, and surface waters. Static tests might not be applicable to materials that are highly volatile, are rapidly biologically or chemically transformed in aqueous solutions, or are removed from test solutions in substantial quantities by the test vessels or organisms during the test. However, practical flow-through test procedures with microalgae have not been developed.

1.4 Results of tests using microalgae should usually be reported in terms of the 96-h (or other time period) IC50 (see ) based on reduction in growth. In some situations, it might only be necessary to determine whether a specific concentration unacceptably affects the growth of the test species or whether the IC50 is above or below a specific concentration.

1.5 This guide is arranged as follows:

This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Specific hazard statements are given in Section 7.

2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

ASTM Standards

D1129 Terminology Relating to Water

D3731 Practices for Measurement of Chlorophyll Content of Algae in Surface Waters

D3978 Practice for Algal Growth Potential Testing with Pseudokirchneriella subcapitata

D4447 Guide for Disposal of Laboratory Chemicals and Samples

E729 Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians

E943 Terminology Relating to Biological Effects and Environmental Fate

E1023 Guide for Assessing the Hazard of a Material to Aquatic Organisms and Their Uses

E1192 Guide for Conducting Acute Toxicity Tests on Aqueous Ambient Samples and Effluents with Fishes, Macroinvertebrates, and Amphibians

E1733 Guide for Use of Lighting in Laboratory Testing

E1847 Practice for Statistical Analysis of Toxicity Tests Conducted Under ASTM Guidelines

Keywords

algae; algal-toxicity testing; aquatic plants; aquatic toxicity testing; microalgae;

ICS Code

ICS Number Code 07.100.20 (Microbiology of water)

DOI: 10.1520/E1218-04E01

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