eLibrary >
PD ISO/TS 21569-3:2015 Horizontal methods for molecular biomarker analysis. Methods of analysis for the detection of genetically modified organisms and derived products - Construct-specific real-time PCR method for detection of P35S-pat-sequence for scree >
Expand All
TOC Search:
PD ISO/TS 21569-3:2015 Horizontal methods for molecular biomarker analysis. Methods of analysis for the detection of genetically modified organisms and derived products - Construct-specific real-time PCR method for detection of P35S-pat-sequence for scree, 2015
- Section sec_1
- Section sec_2
- Section sec_3
- Section sec_4
- Section sec_5
- Section sec_5.1
- Section sec_5.2
- Section sec_5.2.1
- Section sec_5.2.2
- Section sec_5.2.3
- Table tab_1
- Section sec_5.2.4
- Section sec_6
- Section sec_6.1
- Section sec_7
- Section sec_7.1
- Section sec_7.2
- Section sec_7.3
- Table tab_2
- Section sec_7.4
- Table tab_3
- Section sec_8
- Section sec_8.1
- Section sec_8.2
- Section sec_8.3
- Section sec_9
- Section sec_9.1
- Section sec_9.2
- Table tab_4
- Table tab_5
- Section sec_9.3
- Table tab_6
- Table tab_7
- Section sec_9.4
- Section sec_9.5
- Section sec_10
- Reference ref_1
- Reference ref_2
- Reference ref_3
- Reference ref_4
- Reference ref_5
- Reference ref_6
- Reference ref_7
- Reference ref_8
- Reference ref_9
- Reference ref_10
- Foreword
- 1 Scope
- 2 Normative references
- 3 Terms and definitions
- 4 Principle
- 5 Reagents and materials
- 5.1 General
- 5.2 PCR reagents
- 6 Apparatus
- 7 Procedure
- 7.1 Test sample preparation
- 7.2 Preparation of the DNA extracts
- 7.3 PCR setup
- 7.4 Temperature-time programme
- 8 Accept/reject criteria
- 8.1 General
- 8.2 Identification
- 8.3 Calculation of P35S-pat copy numbers
- 9 Validation status and performance criteria
- 9.1 Robustness of the method
- 9.2 Collaborative trial for determination of LOD
- 9.3 Collaborative trial for quantification of the P35S-pat construct in rapeseed
- 9.4 Sensitivity
- 9.5 Specificity
- 10 Test report
- Bibliography [Go to Page]