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BS ISO 23443:2020 Infant formula and adult nutritionals. Determination of β-carotene, lycopene and lutein by reversed-phase ultra-high performance liquid chromatography (RP-UHPLC) >
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BS ISO 23443:2020 Infant formula and adult nutritionals. Determination of β-carotene, lycopene and lutein by reversed-phase ultra-high performance liquid chromatography (RP-UHPLC), 2020
- undefined
- Foreword
- Introduction
- 1 Scope
- 2 Normative references
- 3 Terms and definitions
- 4 Principle
- 5 Reagents and materials
- 5.1 Reagents
- 5.2 Standards
- 5.3 Standards preparation
- 6 Apparatus
- 7 Procedure
- 7.1 Sample preparation
- 7.2 Chromatography
- 7.2.1 Chromatographic conditions
- 7.2.2 System suitability checks
- 8 Calculations
- 8.1 Determination of purity
- 8.1.1 General
- 8.1.2 Spectrophotometric purity (PS)
- 8.1.3 Chromatographic purity (PC)
- 8.1.4 Reference standard purity (P)
- 8.2 Concentration of each carotenoid in standard solutions
- 8.2.1 Stock solution concentrations
- 8.2.2 Apocarotenal working solution concentration
- 8.2.3 Apocarotenal intermediate solution concentration
- 8.2.4 Carotenoid concentrations in mixed carotenoid intermediate solution
- 8.2.5 Concentrations of carotenoid analytes in calibrations solutions
- 8.2.6 Concentration of apocarotenal internal standard in calibrations solutions
- 8.3 Calculate calibration curve
- 8.4 Mass of apocarotenal
- 8.5 Contents of all-trans-β-carotene, cis isomers of β-carotene and total β-carotene
- 8.6 Contents of total lycopene
- 9 Precision
- 9.1 General
- 9.2 Repeatability
- 9.3 Reproducibility
- 10 Test report
- Annex A (informative) Example chromatograms
- Annex B (informative) Precision data
- Annex C (informative) Determination of lutein
- Bibliography [Go to Page]